STELLARIS STED (Confocal + STED Super-Resolution)
The STELLARIS STED is used for confocal, super‐resolution or nanoscopy. It is an inverted microscope, capable of imaging live cells as well as a variety of specimens (ie. fixed, live, thin, thick, etc.) and a variety of fluorophores due to the extraordinary versatility of the system. For instance, the STELLARIS STED includes:
- Tunable excitation with the White Light Laser (485‐685nm)
- Tunable beam splitter able to produce 8 laser lines at once.
- Filter‐free and tunable detection from 410‐850nm for all detectors
- 5 ultra‐sensitive HyD (Hybrid) detectors with high Quantum Efficiency, extremely short dead time (<1.6ns) and photon counting capabilities
- Utilization of the photon arrival time (on‐the‐fly) during acquisition with the FLIM (tauSense)
- All of which applies to confocal, super‐resolution with LIGHTNING as well as nanoscopy with tauSTED
Super‐resolution with LIGHTNING relies on high quality images with the ultra‐sensitive HyD detectors combined with robust, adaptive algorithms to extract information for 120nm xy resolution and <300nm axial resolution.
STED microscopy is a purely optical super‐resolution technique and able to achieve (theoretically) resolutions <30nm in xy and <100nm axial resolution. STED microscopy is suitable for a wide variety of specimens ranging from monolayers of cells to thicker tissues and is compatible with a variety of fluorophores.
Features:
- The STELLARIS STED is integrated with the Leica DMi8, which is a fully motorized and automated inverted microscope stand
- Includes motorized xy scanning stage with a travel range of at least 120 x 80mm and resolution <0.05mm
- Fully motorized DIC, including objective prisms that automatically move out to ensure the most efficient light paths is always used
- Entirely filter‐free and tunable (410‐850nm) with respect to the detection for all imaging modalities (ie. confocal, super‐resolution and nanoscopy)
- Includes he Super Z Galvo Focusing Stage with a travel range of at least 1500 mm
- Equipped with Adaptive Focus Control (AFC), which is a hardware‐based mechanism used to maintain focus on the coverglass
- Configured with a White Light Laser (WLL), which is a super‐continuum laser providing 200 laser lines ranging from 485nm to 685nm, in addition to 405nm laser
- Configured with Acousto‐Optical Beam Splitter (AOBS) to efficiently access all 200 laser lines of the WILL and allow up to 8 laser lines be used simultaneously
- Configured with 5 separate HyD (HyD S and HyD X) detectors for fluorescence imaging
- Includes the LIGHTNING module for super-resolution imaging (resolution down to 120nm xy and <300nm in z) as well as enhanced signal-to-noise ratio
- Includes objectives: PL APO 10x/0.40, PL APO 20x/0.75 (water, glycerol and oil), HC PL APO 40x/1.10 W, PL APO 63x/1.40 oil, PL APO 93x/1.30 GLY motCORR STED WHITE, HC PL APO 100x/1,4 oil STED WHITE
- Nanoscopy with STED microscopy capabilities include:
STED microscopy is the only purely optical super‐resolution technique and with the Leica STELLARIS STED offering:- Tunable resolution from confocal to <50nm in xy and <130nm in z
- Tunable excitation with the White light laser
- Filter‐free and tunable detection
- Ultra‐sensitive HyD detectors
- 592nm and 775nm depletion lasers
- CW STED, gatedSTED, pulsed STED, tauSTED
- tauSTED utilizes the photon arrival time on‐the‐fly during acquisition enabling nanoscopy with less depletion laser power compared to the “traditional” approaches
- Specialized objectives specifically designed for STED microscopy
- Compatible with fixed and live specimens, thick and thin specimens and a variety of fluorophores for multi‐colour imaging
