UltraMicroscope Blaze II Lightsheet
The UltraMicroscope Blaze II is a fully automated lightsheet microscope for imaging large or multiple cleared samples at subcellular resolution.
The large sample holder can hold multiple samples at once, which can then be imaged sequentially and effortlessly (including an entire mouse, rat or marmoset brain).
The UltraMicroscope Blaze™ Imaging System uses cutting-edge illumination optics to slightly tilt 2×3 bidirectional light sheets, with their Rayleigh lengths overlapping in the entire field of view. All six light sheets converge on the focal plane to illuminate all areas of the sample and minimize shadow artifacts. Improved optional sectioning gets the most out of the sample.
Features
- Fully automated light sheet microscope for cleared samples
- One lightsheet thickness: <4.5 μm @ 488 nm
- Focus range: > ±5 mm from center position of sample for all imaging solutions
- Motorized detection module for up to three MI Plan objective lenses
- Motorized: z-drive, autofocus, filter wheel for up to seven 43 mm filters, objective turret, tube lens magnification change unit (0.6x, 1x, 1.67x and 2.5x)
- A motorized cuvette release enables easy sample access. Sample holder travel range: x=24mm, y=50mm, z=23mm
Specifications
- Illumination Uni- and bi-directional
- Number of light sheet 1–6
- Thickness 4–24 µm
- Width 1–20 mm
- Numerical aperture 0.0135–0.135
- Focus positioning Dynamic
- Objective lenses 1.1× 4× 12×
- Zoom | magnification changer 0.66–2.75× 2.4–10× 7.2–30×
- Numerical aperture 0.1 0.35 0.53
- Max. theoretical resolution at detector 4.8 µm 1.3 µm 0.5 µm
- Working distance ≤17 mm ≤16 mm ≤10.9 mm
- FOV diagonal (5.5 MP camera) 7.9–33 mm 2.2–9.1 mm 0.73–3 mm
- Imaging solution Aqueous buffers and organic solvents
- Batch imaging mode Multiple samples can be imaged in one session.
- Refractive index 1.33 (Water)–1.56 (DBA) – Different dipping caps
- UMM lasers 488 nm, 561 nm, 639 nm, 785 nm